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Nat Commun. 2018 Oct 2;9(1):4031. doi: 10.1038/s41467-018-06404-w.

LOTUS domain protein MARF1 binds CCR4-NOT deadenylase complex to post-transcriptionally regulate gene expression in oocytes.

Author information

1
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, 521 A Physiology Building, Baltimore, MD, 21205, USA.
2
Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, 521 A Physiology Building, Baltimore, MD, 21205, USA. fukunaga@jhmi.edu.

Abstract

Post-transcriptional regulation of gene expression plays an essential role during oocyte maturation. Here we report that Drosophila MARF1 (Meiosis Regulator And mRNA Stability Factor 1), which consists of one RNA-recognition motif and six tandem LOTUS domains with unknown molecular function, is essential for oocyte maturation. When tethered to a reporter mRNA, MARF1 post-transcriptionally silences reporter expression by shortening reporter mRNA poly-A tail length and thereby reducing reporter protein level. This activity is mediated by the MARF1 LOTUS domain, which binds the CCR4-NOT deadenylase complex. MARF1 binds cyclin A mRNA and shortens its poly-A tail to reduce Cyclin A protein level during oocyte maturation. This study identifies MARF1 as a regulator in oocyte maturation and defines the conserved LOTUS domain as a post-transcriptional effector domain that recruits CCR4-NOT deadenylase complex to shorten target mRNA poly-A tails and suppress their translation.

PMID:
30279526
PMCID:
PMC6168497
DOI:
10.1038/s41467-018-06404-w
[Indexed for MEDLINE]
Free PMC Article

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