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Biomed Res Int. 2014;2014:934848. doi: 10.1155/2014/934848. Epub 2014 Aug 4.

Proteomics and metabolomics for in situ monitoring of wound healing.

Author information

1
Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany.
2
University Center of Orthopedics and Trauma Surgery, University Hospital "Carl Gustav Carus", TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
3
Department of Oral and Maxillofacial Surgery, University Hospital "Carl Gustav Carus", TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
4
Department of Metabolomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany ; Institute of Pharmacy, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, 04103 Leipzig, Germany.
5
Institute of Physiological Chemistry, TU Dresden, Fiedlerstraße 42, 01307 Dresden, Germany.
6
Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany ; Department of Metabolomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany ; Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, 9000 Aalborg, Denmark.

Abstract

Wound healing of soft tissue and bone defects is a complex process in which cellular differentiation and adaption are regulated by internal and external factors, among them are many different proteins. In contrast to insights into the significance of various single proteins based on model systems, the knowledge about the processes at the actual site of wound healing is still limited. This is caused by a general lack of methods that allow sampling of extracellular factors, metabolites, and proteins in situ. Sampling of wound fluids in combination with proteomics and metabolomics is one of the promising approaches to gain comprehensive and time resolved data on effector molecules. Here, we describe an approach to sample metabolites by microdialysis and to extract proteins simultaneously by adsorption. With this approach it is possible (i) to collect, enrich, and purify proteins for a comprehensive proteome analysis; (ii) to detect more than 600 proteins in different defects including more than 100 secreted proteins, of which many proteins have previously been demonstrated to have diagnostic or predictive power for the wound healing state; and (iii) to combine continuous sampling of cytokines and metabolites and discontinuous sampling of larger proteins to gain complementary information of the same defect.

PMID:
25162036
PMCID:
PMC4137721
DOI:
10.1155/2014/934848
[Indexed for MEDLINE]
Free PMC Article

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