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Mol Cell Biol. 2014 Jun;34(12):2339-49. doi: 10.1128/MCB.00042-14. Epub 2014 Apr 14.

KSRP and MicroRNA 145 are negative regulators of lipolysis in white adipose tissue.

Author information

1
Department of Biochemistry & Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama, USA.
2
Gene Expression Regulation Laboratory, IRCCS AOU San Martino-IST, Genoa, Italy.
3
Department of Biochemistry & Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama, USA cchen@uab.edu.

Abstract

White adipose tissue (WAT) releases fatty acids from stored triacylglycerol for an energy source. Here, we report that targeted deletion of KH-type splicing regulatory protein (KSRP), an RNA-binding protein that regulates gene expression at multiple levels, enhances lipolysis in epididymal WAT (eWAT) because of the upregulation of genes promoting lipolytic activity. Expression of microRNA 145 (miR-145) is decreased because of impaired primary miR-145 processing in Ksrp(-/-) eWAT. We show that miR-145 directly targets and represses Foxo1 and Cgi58, activators of lipolytic activity, and forced expression of miR-145 attenuates lipolysis. This study reveals a novel in vivo function of KSRP in controlling adipose lipolysis through posttranscriptional regulation of miR-145 expression.

PMID:
24732799
PMCID:
PMC4054295
DOI:
10.1128/MCB.00042-14
[Indexed for MEDLINE]
Free PMC Article

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