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J Histochem Cytochem. 2019 Nov;67(11):813-824. doi: 10.1369/0022155419871027. Epub 2019 Aug 19.

KIF11 as a Potential Marker of Spermatogenesis Within Mouse Seminiferous Tubule Cross-sections.

Author information

1
Department of Biochemistry, Graduate School of Medical and Dental Sciences,Tokyo Medical and Dental University, Tokyo, Japan.
2
Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan.
3
Faculty of Health Science, Aino University, Osaka, Japan.
4
Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, Chiba, Japan.
5
Plastic Reconstructive & Regenerative Surgery, Nippon Medical School, Tokyo, Japan.
6
Atopy Research Center, Juntendo University Graduate School of Medicine, Tokyo, Japan.
7
Department of Biochemistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
8
Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya, Japan.
9
Department of Biomedical Sciences, Chubu University, Aichi, Japan.

Abstract

The arrangement of immature germ cells changes regularly and periodically along the axis of the seminiferous tubule, and is used to describe the progression of spermatogenesis. This description is based primarily on the changes in the acrosome and the nuclear morphology of haploid spermatids. However, such criteria cannot be applied under pathological conditions with arrested spermatid differentiation. In such settings, the changes associated with the differentiation of premeiotic germ cells must be analyzed. Here, we found that the unique bipolar motor protein, KIF11 (kinesin-5/Eg5), which functions in spindle formation during mitosis and meiosis in oocytes and early embryos, is expressed in premeiotic germ cells (spermatogonia and spermatocytes). Thus, we aimed to investigate whether KIF11 could be used to describe the progression of incomplete spermatogenesis. Interestingly, KIF11 expression was barely observed in haploid spermatids and Sertoli cells. The KIF11 staining allowed us to evaluate the progression of meiotic processes, by providing the time axis of spindle formation in both normal and spermatogenesis-arrested mutant mice. Accordingly, KIF11 has the potential to serve as an excellent marker to describe spermatogenesis, even in the absence of spermatid development.

KEYWORDS:

meiosis; spermatogenesis; testis

PMID:
31424977
PMCID:
PMC6824007
[Available on 2020-11-01]
DOI:
10.1369/0022155419871027

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