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See 1 citation in J Clin Endocrinol Metab 2017:

J Clin Endocrinol Metab. 2017 Jul 1;102(7):2564-2574. doi: 10.1210/jc.2016-3591.

Impaired Release of Vitamin D in Dysfunctional Adipose Tissue: New Cues on Vitamin D Supplementation in Obesity.

Author information

Department of Medicine, Unit of Andrology and Reproductive Medicine, University of Padova, 35128 Padova, Italy.
Familial Tumor Unit, Veneto Institute of Oncology Istituto Oncologico Veneto-Istituto di Ricovero e Cura a Carattere Scientifico, 35128 Padova, Italy.
Laboratory of Protein Chemistry, Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35128 Padova, Italy.
Burn Unit and Plastic Surgery, University Hospital of Padova, 35128 Padova, Italy.
Department of Medicine, Internal Medicine, University of Padova, 35128 Padova, Italy.
Department of Medicine, Laboratory Medicine, University of Padova, 35128 Padova, Italy.



Vitamin D accumulates in adipose tissue (AT), and vitamin D deficiency is frequent in obesity.


We hypothesize that trafficking of vitamin D is altered in dysfunctional AT.

Design, Patients, Settings:

Fifty-four normal-weight and 67 obese males were recruited in a prospective study and randomly assigned to supplementation with 50 µg/wk 25-hydroxyvitamin-D3 or 150 µg/wk vitamin D3 for 1 year, raising dosage by 50% if vitamin D sufficiency [serum 25-hydroxyvitamin-D3 >50 nmol/L], was not achieved at 6 months; 97 subjects completed the study.


Vitamin D3 and 25-hydroxyvitamin-D3 were quantified by HPLC-MS in control and insulin-resistant (IR) 3T3-L1 cells and subcutaneous AT (SAT) from lean and obese subjects, incubated with or without adrenaline; expression of 25-hydroxylase (Cyp27a1), 1α-hydroxylase (Cyp27b1), and vitamin D receptor (Vdr) was analyzed by real-time polymerase chain reaction.


In IR adipocytes, uptake of D3 and 25-hydroxyvitamin-D3 was higher, but, after adrenaline stimulation, the decrement in D3 and 25-hydroxyvitamin-D3 was stronger in control cells, which also showed increased expression of Cyp27a1 and Cyp27b1 and higher levels of 25-hydroxyvitamin-D3. In SAT from obese subjects, adrenaline-induced release of D3 and 25-hydroxyvitamin-D3 was blunted; in both IR cells and obese SAT, protein expression of β2-adrenergic receptor was reduced. Supplementation with 25-hydroxyvitamin-D3 was more effective in achieving vitamin D sufficiency in obese, but not in normal weight subjects.


Dysfunctional AT shows a reduced catecholamine-induced release of D3 and 25-hydroxyvitamin-D3 and altered activity of vitamin D-metabolizing enzymes; for these reasons supplementation with 25-hydroxyvitamin-D3 is more effective in obese individuals.

[Indexed for MEDLINE]

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