Format

Send to

Choose Destination

See 1 citation found by title matching your search:

Cytotherapy. 2011 Mar;13(3):341-9. doi: 10.3109/14653249.2010.520312. Epub 2010 Sep 22.

Isolation and characterization of equine amniotic fluid-derived multipotent stem cells.

Author information

1
Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.

Abstract

BACKGROUND AIMS:

Amniotic fluid (AF) is a well-known source of stem cells. However, there have been no reports regarding equine AF stem cells. We have isolated equine AF-derived multipotent stem cells (MSC) (eAF-MSC) and show that these cells exhibit self-renewal ability and multilineage differentiation.

METHODS:

AF was obtained from thoroughbred mares and mononuclear cells (MNC) were isolated by Ficoll-Paque density gradient. We measured the cumulative population doubling level (CPDL) and characterized the immunophenotype by flow cytometry. To investigate differentiation ability, a trilineage differentiation assay was conducted.

RESULTS:

eAF-MSC could be isolated and the proliferation level was high. eAF-MSC presented typical MSC phenotypic markers, as determined by flow cytometry. Moreover, eAF-MSC showed a trilineage differentiation capability.

CONCLUSIONS:

Equine AF is a good source of MSC. Furthermore, eAF-MSC may be useful as a cell therapy application for horses.

PMID:
20860427
DOI:
10.3109/14653249.2010.520312
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center