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Methods Mol Biol. 2018;1829:205-212. doi: 10.1007/978-1-4939-8654-5_14.

Isolating the Plasmodium falciparum Apicoplast Using Magnetic Beads.

Author information

1
ApicoLipid Team, Institute for Advanced Biosciences, CNRS UMR5309, Université Grenoble Alpes, INSERM U1209, Grenoble, France. cyrille.botte@univ-grenoble-alpes.fr.
2
School of Biosciences, University of Melbourne, Melbourne, VIC, Australia.
3
ApicoLipid Team, Institute for Advanced Biosciences, CNRS UMR5309, Université Grenoble Alpes, INSERM U1209, Grenoble, France.

Abstract

Plastids are key organelles in both photosynthetic and nonphotosynthetic organisms. In photosynthetic organisms, plastids can be readily purified using differential centrifugations due to the high density of photosynthetic membranes or thylakoids. The apicomplexan plastid (the apicoplast) is an essential nonphotosynthetic plastid that lacks thylakoid and was not readily purified using conventional methods. Here, we describe a tractable method to purify intact apicoplasts from Plasmodium falciparum blood stages using magnetic beads and affinity purification.

KEYWORDS:

Apicomplexa; Apicoplast; Magnetic column; Magnetic separation; Plasmodium falciparum; Purification

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