Format

Send to

Choose Destination

See 1 citation in Int J Parasitol Drugs Drug Resist 2016:

Int J Parasitol Drugs Drug Resist. 2016 Dec;6(3):171-178. doi: 10.1016/j.ijpddr.2016.08.002. Epub 2016 Aug 26.

Metabolic profiling and in vitro assessment of anthelmintic fractions of Picria fel-terrae Lour.

Author information

1
Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, 3800, Australia.
2
Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, 3122, Victoria, Australia; Land and Water, Commonwealth Scientific and Industrial Research Organization (CSIRO), Ecosciences Precinct, Dutton Park, 4102, Queensland, Australia.
3
Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, 3010, Victoria, Australia.
4
Sarawak Biodiversity Centre (SBC), KM 20 Jalan Borneo Heights, Semengoh, Locked Bag No. 3032, 93990, Kuching, Sarawak, Malaysia.
5
Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, East Melbourne, 3002, Victoria, Australia.
6
Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, East Melbourne, 3002, Victoria, Australia; The Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, 3052, Victoria, Australia.
7
Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, 3122, Victoria, Australia.
8
Land and Water, Commonwealth Scientific and Industrial Research Organization (CSIRO), Ecosciences Precinct, Dutton Park, 4102, Queensland, Australia.
9
Australian Centre for Research on Separation Science (ACROSS), School of Applied Sciences, RMIT University, Melbourne, 3001, Victoria, Australia.
10
Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, 3800, Australia; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, 3010, Victoria, Australia. Electronic address: peter.boag@monash.edu.

Abstract

Anthelmintic resistance is widespread in gastrointestinal nematode populations, such that there is a consistent need to search for new anthelmintics. However, the cost of screening for new compounds is high and has a very low success rate. Using the knowledge of traditional healers from Borneo Rainforests (Sarawak, Malaysia), we have previously shown that some traditional medicinal plants are a rich source of potential new anthelmintic drug candidates. In this study, Picria fel-terrae Lour. plant extract, which has previously shown promising anthelmintic activities, was fractionated via the use of a solid phase extraction cartridge and each isolated fraction was then tested on free-living nematode Caenorhabditis elegans and the parasitic nematode Haemonchus contortus. We found that a single fraction was enriched for nematocidal activity, killing ≥90% of C. elegans adults and inhibiting the motility of exsheathed L3 of H. contortus, while having minimal cytotoxic activity in mammalian cell culture. Metabolic profiling and chemometric analysis of the effective fraction indicated medium chained fatty acids and phenolic acids were highly represented.

KEYWORDS:

Caenorhabditis elegans; Haemonchus contortus; Metabolomics; Picria fel-terrae Lour.; Traditional medicines

PMID:
27639945
PMCID:
PMC5030326
DOI:
10.1016/j.ijpddr.2016.08.002
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center