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Exp Dermatol. 2014 Aug;23(8):596-605. doi: 10.1111/exd.12464.

In vivo study of targeted nanomedicine delivery into Langerhans cells by multiphoton laser scanning microscopy.

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Institute for Solid State Physics and Optics of Wigner RCP, Budapest, Hungary; R&D Ultrafast Lasers Ltd, Budapest, Hungary.


Epidermal Langerhans cells (LCs) function as professional antigen-presenting cells of the skin. We investigated the LC-targeting properties of a special mannose-moiety-coated pathogen-like synthetic nanomedicine DermaVir (DV), which is capable to express antigens to induce immune responses and kill HIV-infected cells. Our aim was to use multiphoton laser microscopy (MLM) in vivo in order to visualize the uptake of Alexa-labelled DV (AF546-DV) by LCs. Knock-in mice expressing enhanced green fluorescent protein (eGFP) under the control of the langerin gene (CD207) were used to visualize LCs. After 1 h, AF546-DV penetrated the epidermis and entered the eGFP-LCs. The AF546-DV signal was equally distributed inside the LCs. After 9 h, we observed AF546-DV signal accumulation that occurred mainly at the cell body. We demonstrated in live animals that LCs picked up and accumulated the nanoparticles in the cell body.


Langerhans cells; eGFP-Langerin knock-in mice; in vivo; multiphoton laser microscopy; nanomedicine formulation

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