Format

Send to

Choose Destination

See 1 citation found by title matching your search:

Blood. 2018 Aug 2;132(5):e1-e12. doi: 10.1182/blood-2017-12-822890. Epub 2018 May 21.

Identification of key lipids critical for platelet activation by comprehensive analysis of the platelet lipidome.

Author information

1
Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Dortmund, Germany.
2
Department of Cardiology and Cardiovascular Medicine and.
3
Department of Physiology, University of Tübingen, Tübingen, Germany.
4
Medizinische Fakultät, Ruhr-Universität Bochum, Bochum, Germany; and.
5
College of Physical Sciences, University of Aberdeen, Old Aberdeen, United Kingdom.

Abstract

Platelet integrity and function critically depend on lipid composition. However, the lipid inventory in platelets was hitherto not quantified. Here, we examined the lipidome of murine platelets using lipid-category tailored protocols on a quantitative lipidomics platform. We could show that the platelet lipidome comprises almost 400 lipid species and covers a concentration range of 7 orders of magnitude. A systematic comparison of the lipidomics network in resting and activated murine platelets, validated in human platelets, revealed that <20% of the platelet lipidome is changed upon activation, involving mainly lipids containing arachidonic acid. Sphingomyelin phosphodiesterase-1 (Smpd1) deficiency resulted in a very specific modulation of the platelet lipidome with an order of magnitude upregulation of lysosphingomyelin (SPC), and subsequent modification of platelet activation and thrombus formation. In conclusion, this first comprehensive quantitative lipidomic analysis of platelets sheds light on novel mechanisms important for platelet function, and has therefore the potential to open novel diagnostic and therapeutic opportunities.

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center