(a) Immunostaining with consecutive sections on Tx-d3. Left panel: double staining for vWF (ECs, green) and cTnT (CMs, red), and DAPI. Right panel: double staining for vWF (ECs, green) and HNA (human cell nuclei, red), and DAPI. (b,c) Immunostaining on Tx-d28. (b) Double staining for cTnT (CMs, green) and HNA (human cell nuclei, red), and DAPI. Upper panels: representative staining for engrafted area. Right panel: higher magnification view of left panel (square). Lower panel: quantification of engrafted area. The ratio of the engrafted area (area in the broken magenta line) and the MI area (area in the broken white line) is shown. (c) Double staining for vWF (ECs, green) and HNA (human cell nuclei, red), and DAPI. Tx-d3 and d28: 3 and 28 days after Tx, respectively. Scale bars: 2 mm in b (lower panel), 500 μm in b (upper left panel), 200 μm in a, and 100 μm in b (upper right panel) and c.