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Chem Commun (Camb). 2014 Mar 25;50(24):3159-62. doi: 10.1039/c4cc00070f. Epub 2014 Jan 29.

Highly efficient one-pot multienzyme (OPME) synthesis of glycans with fluorous-tag assisted purification.

Author information

1
Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA. xiichen@ucdavis.edu.

Abstract

Oligo(ethylene glycol)-linked light fluorous tags have been found to be optimal for conjugating to glycans for both high-yield enzymatic glycosylation reactions using one-pot multienzyme (OPME) systems and quick product purification using fluorous solid-phase extraction (FSPE) cartridges. The combination of OPME glycosylation systems and the FSPE cartridge purification scheme provides a highly effective strategy for facile synthesis and purification of glycans.

PMID:
24473465
DOI:
10.1039/c4cc00070f
[Indexed for MEDLINE]

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