High-mobility Group Box 1 Is Associated with the Inflammatory Infiltration and Alveolar Bone Destruction in Rats Experimental Periapical Lesions

J Endod. 2017 Jun;43(6):964-969. doi: 10.1016/j.joen.2016.11.014. Epub 2017 Apr 4.

Abstract

Introduction: This study was conducted to observe the immunohistochemical localization of high-mobility group box 1 (HMGB1) and its receptor, Toll-like receptor 4 (TRL4), in the development of periapical lesions induced in rats. The possible role of these molecules in the pathogenesis of periapical lesions was also explored.

Methods: Periapical lesions developed within 35 days after mandibular first molar pulp exposure in Wistar rats. The animals were randomly killed at 0, 7, 14, 21, 28, and 35 days after pulp exposure. The jaws that contained the first molar were obtained and prepared for histologic analysis, enzyme histochemistry, immunohistochemistry, and double immunofluorescence staining.

Results: From day 0 to 35, the areas of periapical bone loss increased and appeared to be stabilized on day 35. A few HMGB1-positive, TLR4-positive cells and osteoclasts could be observed on day 7. From day 7 to 28, the HMGB1 and TLR4 protein expression increased and subsequently remained stable. The number of osteoclasts multiplied from day 0 to 14 and then gradually decreased from day 14 to 35. Double immunofluorescence staining results showed HMGB1-positive, TLR4-positive cells around periapical lesions surrounding the apical foramen.

Conclusions: Thus, HMGB1 and TLR4 may be associated with the pathogenesis of the periapical lesions.

Keywords: High-mobility group box 1; osteoclast; periapical lesions; toll-like receptor 4.

MeSH terms

  • Alveolar Bone Loss / metabolism*
  • Alveolar Bone Loss / pathology
  • Animals
  • Fluorescent Antibody Technique
  • HMGB1 Protein / metabolism*
  • Inflammation / metabolism
  • Male
  • Periapical Diseases / metabolism*
  • Periapical Diseases / pathology
  • Rats
  • Rats, Wistar
  • Toll-Like Receptor 4 / metabolism

Substances

  • HMGB1 Protein
  • Tlr4 protein, rat
  • Toll-Like Receptor 4