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Stroke. 1995 Dec;26(12):2313-9; discussion 2319-20.

HU-211, a novel noncompetitive N-methyl-D-aspartate antagonist, improves neurological deficit and reduces infarct volume after reversible focal cerebral ischemia in the rat.

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1
Department of Neurology, University of Miami School of Medicine, FL 33101, USA.

Abstract

BACKGROUND AND PURPOSE:

HU-211 is a nonpsychotropic cannabinoid analogue that has been shown to act as a functional N-methyl-D-aspartate receptor blocker. We investigated the neuroprotective efficacy of HU-211 in a model of reversible middle cerebral artery occlusion (MCAo) in rats.

METHODS:

Male Wistar rats were anesthetized with halothane and subjected to 90 minutes of temporary MCAo by retrograde insertion of an intraluminal nylon suture, coated with poly-L-lysine, through the external carotid artery into the internal carotid artery and MCA. The drug (HU-211 in cosolvent, 4 mg/kg IV) or vehicle was administered in a blinded fashion 70 minutes after onset of MCAo. Behavioral tests were evaluated during occlusion (60 minutes) and for a 3-day period after MCAo. Three days after MCAo, brains were perfusion-fixed, and infarct volumes were determined.

RESULTS:

HU-211 significantly improved the neurological score compared with vehicle during the 3 days after MCAo. Treatment with HU-211 also significantly reduced both infarct volume (mean +/- SEM, 66.6 +/- 12.5 versus 149.8 +/- 36.3 mm3) and brain swelling (2.61 +/- 1.33% versus 6.66 +/- 1.24%) compared with vehicle-treated rats (n = 17 in each group).

CONCLUSIONS:

These results demonstrate the neuroprotective ability of HU-211 in focal cerebral ischemia as judged by neurological score, infarct size, and brain swelling. Reversible MCAo with the use of a poly-L-lysine-coated intraluminal suture proved to be a reliable and effective modification of this technique, yielding consistent results.

PMID:
7491657
[Indexed for MEDLINE]

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