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AIDS Res Hum Retroviruses. 2017 Aug;33(8):859-868. doi: 10.1089/AID.2016.0294. Epub 2017 May 30.

HIV-1 Consensus Envelope-Induced Broadly Binding Antibodies.

Author information

1
1 Duke Human Vaccine Institute, Duke University School of Medicine , Durham, North Carolina.
2
2 Department of Immunology, Duke University School of Medicine , Durham, North Carolina.
3
3 Department of Surgery, Duke University School of Medicine , Durham, North Carolina.
4
4 College of Life Science and Technology, Jinan University , Guangzhou, China .
5
5 Los Alamos National Laboratory , Los Alamos, New Mexico .
6
6 Department of Medicine, Duke University School of Medicine , Durham, North Carolina.

Abstract

Antibodies that cross-react with multiple HIV-1 envelopes (Envs) are useful reagents for characterizing Env proteins and for soluble Env capture and purification assays. We previously reported 10 murine monoclonal antibodies induced by group M consensus Env, CON-6 immunization. Each demonstrated broad cross-reactivity to recombinant Envs. Here we characterized the Env epitopes to which they bind. Seven mapped to linear epitopes in gp120, five at the Env N-terminus, and two at the Env C-terminus. One antibody, 13D7, bound at the gp120 N-terminus (aa 30-42), reacted with HIV-1-infected CD4+ T cells, and when expressed in a human IgG1 backbone, mediated antibody-dependent cellular cytotoxicity. Antibody 18F11 bound at the gp120 C-terminus (aa 445-459) and reactivity was glycan dependent. Antibodies 13D7, 3B3, and 16H3 bound to 100 percent of HIV-1 Envs tested in ELISA and sodium dodecyl sulfate/polyacrylamide gel electrophoresis/western blot analysis. These data define the epitopes of monoclonal antibody reagents for characterization of recombinant Envs, one epitope of which is also expressed on the surface of HIV-1-infected CD4+ T cells.

KEYWORDS:

ADCC; HIV; antibody-mediated immunity; monoclonal; vaccine design

PMID:
28314374
PMCID:
PMC5564029
DOI:
10.1089/AID.2016.0294
[Indexed for MEDLINE]
Free PMC Article

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