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Appl Microbiol Biotechnol. 2006 Jul;71(3):323-8. Epub 2006 Apr 28.

Growth of Escherichia coli MG1655 on LB medium: determining metabolic strategy with transcriptional microarrays.

Author information

1
Integrated Genomics Incorporated, Chicago, IL 60612, USA. mark@integratedgenomics.com

Abstract

Expression profiles of genes related to stress responses, substrate assimilation, acetate metabolism, and biosynthesis were obtained by monitoring growth of Escherichia coli MG1655 in Luria-Bertani (LB) medium with transcriptional microarrays. Superimposing gene expression profiles on a plot of specific growth rate demonstrates that the cells pass through four distinct physiological states during fermentation before entering stationary phase. Each of these states can be characterized by specific patterns of substrate utilization and cellular biosynthesis corresponding to the nutrient status of the medium. These data allow the growth phases of the classical microbial growth curve to be redefined in terms of the physiological states and environmental changes commonly occurring during bacterial growth in batch culture on LB medium.

PMID:
16645822
DOI:
10.1007/s00253-006-0392-8
[Indexed for MEDLINE]

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