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Plant Biotechnol J. 2015 Sep;13(7):993-1001. doi: 10.1111/pbi.12342. Epub 2015 Feb 3.

Genetic engineering of the Xa10 promoter for broad-spectrum and durable resistance to Xanthomonas oryzae pv. oryzae.

Zeng X1,2, Tian D1, Gu K1, Zhou Z1, Yang X1, Luo Y1,3, White FF4, Yin Z1,2,5.

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Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Rice Research Institute, Anhui Academy of Agricultural Sciences, Anhui, China.
Department of Plant Pathology, Kansas State University, Manhattan, KS, USA.
School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.


Many pathovars of plant pathogenic bacteria Xanthomonas species inject transcription activator-like (TAL) effectors into plant host cells to promote disease susceptibility or trigger disease resistance. The rice TAL effector-dependent disease resistance gene Xa10 confers narrow-spectrum race-specific resistance to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight disease in rice. To generate broad-spectrum and durable resistance to Xoo, we developed a modified Xa10 gene, designated as Xa10(E5) . Xa10(E5) has an EBE-amended promoter containing 5 tandemly arranged EBEs each responding specifically to a corresponding virulent or avirulent TAL effector and a stable transgenic rice line containing Xa10(E5) was generated in the cultivar Nipponbare. The Xa10(E5) gene was specifically induced by Xoo strains that harbour the corresponding TAL effectors and conferred TAL effector-dependent resistance to the pathogens at all developmental stages of rice. Further disease evaluation demonstrated that the Xa10(E5) gene in either Nipponbare or 9311 genetic backgrounds provided broad-spectrum disease resistance to 27 of the 28 Xoo strains collected from 11 countries. The development of Xa10(E5) and transgenic rice lines provides new genetic materials for molecular breeding of rice for broad-spectrum and durable disease resistance to bacterial blight.


R gene; Rice; TAL effector; Xa10; Xanthomonas oryzae pv. oryzae; bacterial blight

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