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Physiol Rep. 2017 Jun;5(12). pii: e13328. doi: 10.14814/phy2.13328.

Highly suggestive preliminary evidence that the renal interstitium contracts in vivo.

Author information

1
Integrative Physiology Laboratory, Department of Physiology and Biophysics, Autonomous University of San Luis Potosí, México.
2
Renal Laboratory, Faculty of Medicine, Autonomous University of San Luis Potosí, México.
3
Integrative Physiology Laboratory, Department of Physiology and Biophysics, Autonomous University of San Luis Potosí, México rodrigum@uaslp.mx.

Abstract

To learn more about controlling renal interstitial hydrostatic pressure (RIHP), we assessed its response to renal medullary direct interstitial volume expansion (rmDIVE = 100 μL bolus infusion/30 sec). Three experimental series (S) were performed in hydropenic, anesthetized, right-nephrectomized, acute left renal-denervated and renal perfusion pressure-controlled rats randomly assigned to groups in each S. S1: Rats without hormonal clamp were contrasted before and after rmDIVE induced via 0.9% saline solution bolus (SS group) or 2% albumin in SS bolus (2% ALB + SS group). Subcapsular ΔRIHP rose slowly, progressively and similarly in both groups by ~3 mmHg. S2: Rats under hormonal clamp were contrasted before and after sham rmDIVE (time CTR group) and real rmDIVE induced via either SS bolus (SS group) or SS bolus containing the subcutaneous tissue fibroblast relaxant dibutyryl-cAMP (SS + db-cAMP group). ΔRIHP showed time, group, and time*group interaction effects with a biphasic response (early: ~1 mmHg; late: ~4 mmHg) in the SS group that was absent in the SS + db-cAMP group. S3: Two groups of rats (SS and SS + db-cAMP) under hormonal clamp were contrasted as in S2, producing similar ΔRIHP results to those of S2 but showing a slow, progressive, and indistinct decrease in renal outer medullary blood flow in both groups. These results provide highly suggestive preliminary evidence that the renal interstitium is capable of contracting reactively in vivo in response to rmDIVE with SS and demonstrate that such a response is abolished when db-cAMP is interstitially and concomitantly infused.

KEYWORDS:

db‐cAMP; direct interstitial volume expansion; renal interstitial hydrostatic pressure

PMID:
28646097
PMCID:
PMC5492211
DOI:
10.14814/phy2.13328
[Indexed for MEDLINE]
Free PMC Article

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