Format

Send to

Choose Destination

See 1 citation found by title matching your search:

Int J Parasitol. 2015 Sep;45(11):679-84. doi: 10.1016/j.ijpara.2015.05.001. Epub 2015 Jun 19.

First isolation of a new species of Leishmania responsible for human cutaneous leishmaniasis in Ghana and classification in the Leishmania enriettii complex.

Author information

1
Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, UK; Department of Biomedical and Forensic Sciences, School of Biological Sciences, University of Cape Coast, Cape Coast, Ghana.
2
U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana; Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
3
U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
4
Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, UK.
5
Department of Microbiology, University of Ghana Medical School, Ghana.
6
Ghana Health Service, Accra, Ghana.
7
Disease Control Unit, Ministry of Health, Ho, Ghana.
8
School of Allied Health Sciences, College of Health Sciences, University of Ghana, Korle-Bu, Accra, Ghana.
9
Department of Microbiology, University of Ghana Medical School, Ghana; School of Allied Health Sciences, College of Health Sciences, University of Ghana, Korle-Bu, Accra, Ghana.
10
Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
11
Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, UK. Electronic address: p.bates@lancaster.ac.uk.

Abstract

An active case detection approach with PCR diagnosis was used in the Ho District of the Volta Region, Ghana that identified individuals with active cutaneous leishmaniasis. Three isolates were successfully cultured and DNA sequences from these were analysed (ribosomal RNA internal transcribed spacer 1; ribosomal protein L23a intergenic spacer; RNA polymerase II large subunit), showing them to be Leishmania, identical to each other but different from all other known Leishmania spp. Phylogenetic analysis showed the parasites to be new members of the Leishmania enriettii complex, which is emerging as a possible new subgenus of Leishmania parasites containing human pathogens.

KEYWORDS:

Cutaneous leishmaniasis; Ghana; Leishmania; Phylogeny

PMID:
26099650
DOI:
10.1016/j.ijpara.2015.05.001
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center