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See 1 citation in Fertil Steril 2008:

Fertil Steril. 2008 Nov;90(5):1925-30. Epub 2007 Oct 1.

Modulation of the BCL-2/BAX ratio by interferon-gamma and hypoxia in human peritoneal and adhesion fibroblasts.

Author information

1
Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, CS Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan 48201, USA.

Abstract

OBJECTIVE:

To examine the effect of interferon (IFN)-gamma treatment under normal and hypoxic conditions on the BCL-2/BAX ratio of fibroblasts obtained from normal peritoneal and adhesion tissues of the same patients.

DESIGN:

Prospective experimental study.

SETTING:

University medical center.

PATIENT(S):

Fibroblasts from peritoneum and adhesion tissues of 5 patients.

INTERVENTION(S):

Hypoxia and IFN-gamma treatments of fibroblasts.

MAIN OUTCOME MEASURE(S):

We used the multiplex polymerase chain reaction technique to measure expression of BCL-2 and BAX in normal peritoneal and adhesion fibroblasts exposed to hypoxia (2% O(2)), in the presence or absence of IFN-gamma for different time points and dosages.

RESULT(S):

At baseline, adhesion fibroblasts manifested decreased basal levels of apoptosis compared with normal fibroblasts. Hypoxia treatment resulted in a time-response decrease in apoptosis in both cell lines. Interferon-gamma treatment resulted in a dose-response increase in apoptosis in both cell lines. Hypoxia had a reduced or no effect on apoptosis in the presence of increasing doses of IFN-gamma in both cell types.

CONCLUSION(S):

Interferon-gamma can block the effects of hypoxia on apoptosis, supporting the antifibrogenic nature of this cytokine. This suggests that IFN-gamma would be a good candidate for consideration for intervention in the development of peritoneal adhesions and fibrosis.

[Indexed for MEDLINE]

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