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See 1 citation in Fertil Steril 2008:

Fertil Steril. 2008 Nov;90(5):1925-30. Epub 2007 Oct 1.

Modulation of the BCL-2/BAX ratio by interferon-gamma and hypoxia in human peritoneal and adhesion fibroblasts.

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Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, CS Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan 48201, USA.



To examine the effect of interferon (IFN)-gamma treatment under normal and hypoxic conditions on the BCL-2/BAX ratio of fibroblasts obtained from normal peritoneal and adhesion tissues of the same patients.


Prospective experimental study.


University medical center.


Fibroblasts from peritoneum and adhesion tissues of 5 patients.


Hypoxia and IFN-gamma treatments of fibroblasts.


We used the multiplex polymerase chain reaction technique to measure expression of BCL-2 and BAX in normal peritoneal and adhesion fibroblasts exposed to hypoxia (2% O(2)), in the presence or absence of IFN-gamma for different time points and dosages.


At baseline, adhesion fibroblasts manifested decreased basal levels of apoptosis compared with normal fibroblasts. Hypoxia treatment resulted in a time-response decrease in apoptosis in both cell lines. Interferon-gamma treatment resulted in a dose-response increase in apoptosis in both cell lines. Hypoxia had a reduced or no effect on apoptosis in the presence of increasing doses of IFN-gamma in both cell types.


Interferon-gamma can block the effects of hypoxia on apoptosis, supporting the antifibrogenic nature of this cytokine. This suggests that IFN-gamma would be a good candidate for consideration for intervention in the development of peritoneal adhesions and fibrosis.

[Indexed for MEDLINE]

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