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FASEB J. 2014 Aug;28(8):3671-8. doi: 10.1096/fj.14-251520. Epub 2014 Apr 28.

Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice.

Author information

1
Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Cambridge, UK;
2
Iron Metabolism Laboratory, Queensland Institute of Medical Research Berghofer Medical Research Institute, Brisbane, Queensland, Australia; and.
3
Iron Metabolism Laboratory, Queensland Institute of Medical Research Berghofer Medical Research Institute, Brisbane, Queensland, Australia; and School of Chemistry and Molecular Bioscience and School of Medicine, University of Queensland, Brisbane, Queensland, Australia.
4
Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Cambridge, UK; dora.pereira@mrc-hnr.cam.ac.uk.

Abstract

The ferritin core is composed of fine nanoparticulate Fe(3+) oxohydroxide, and we have developed a synthetic mimetic, nanoparticulate Fe(3+) polyoxohydroxide (nanoFe(3+)). The aim of this study was to determine how dietary iron derived in this fashion is absorbed in the duodenum. Following a 4 wk run-in on an Fe-deficient diet, mice with intestinal-specific disruption of the Fpn-1 gene (Fpn-KO), or littermate wild-type (WT) controls, were supplemented with Fe(2+) sulfate (FeSO4), nanoFe(3+), or no added Fe for a further 4 wk. A control group was Fe sufficient throughout. Direct intestinal absorption of nanoFe(3+) was investigated using isolated duodenal loops. Our data show that FeSO4 and nanoFe(3+) are equally bioavailable in WT mice, and at wk 8 the mean ± SEM hemoglobin increase was 18 ± 7 g/L in the FeSO4 group and 30 ± 5 g/L in the nanoFe(3+) group. Oral iron failed to be utilized by Fpn-KO mice and was retained in enterocytes, irrespective of the iron source. In summary, although nanoFe(3+) is taken up directly by the duodenum its homeostasis is under the normal regulatory control of dietary iron absorption, namely via ferroportin-dependent efflux from enterocytes, and thus offers potential as a novel oral iron supplement.

KEYWORDS:

basolateral export; hepcidin; iron homeostasis; knockout mice; nanoiron

PMID:
24776745
PMCID:
PMC4101650
DOI:
10.1096/fj.14-251520
[Indexed for MEDLINE]
Free PMC Article

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