Send to

Choose Destination

See 1 citation found by title matching your search:

See comment in PubMed Commons below
Mol Cell Biochem. 2011 Jan;346(1-2):81-7. doi: 10.1007/s11010-010-0593-4. Epub 2010 Oct 8.

Fatty acylation enhances cellular localization of dansylated phenylalanines.

Author information

Department of Pharmaceutical Sciences, College of Pharmacy, The University of Findlay, 1000 North Main Street, Findlay, OH 45840, USA.


Two series of fluorescent molecules were synthesized by acylation of dansyl ethylenediamine and phenylalanine dansyl ethylenediamine with one of either acetyl (C(2)), hexanyl (C(6)), cyclohexanecarbonyl (C(7)), myristyl (C(14)), or palmityl (C(16)) groups and examined for entry and localization in Chinese Hamster Ovary (CHO) cells in tissue culture. Gross total fluorescence retention and cellular microscopic fluorescence patterns were analyzed. In both series, molecules with myristyl or palmityl groups entered cells. Only in the phenylalanine series did hexyl and cyclohexanecarbonyl modification enable entry. Consistent with a mechanism of passive diffusion, entry of compounds into cells was neither energy dependent nor endocytosis linked. Acylated molecules were observed to localize in cytoplasm and not enter nuclei or associate with lipophilic plasma membranes.

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center