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J Chem Inf Model. 2014 Oct 27;54(10):2887-901. doi: 10.1021/ci5001955. Epub 2014 Sep 18.

Experimentally validated HERG pharmacophore models as cardiotoxicity prediction tools.

Author information

1
Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina , 88.040-900 Florianópolis, Santa Catarina, Brazil.

Abstract

The goal of this study was to design, experimentally validate, and apply a virtual screening workflow to identify novel hERG channel blockers. The hERG channel is an important antitarget in drug development since cardiotoxic risks remain as a major cause of attrition. A ligand-based pharmacophore model collection was developed and theoretically validated. The seven most complementary and suitable models were used for virtual screening of in-house and commercially available compound libraries. From the hit lists, 50 compounds were selected for experimental validation through bioactivity assessment using patch clamp techniques. Twenty compounds inhibited hERG channels expressed in HEK 293 cells with IC50 values ranging from 0.13 to 2.77 μM, attesting to the suitability of the models as cardiotoxicity prediction tools in a preclinical stage.

PMID:
25148533
DOI:
10.1021/ci5001955
[Indexed for MEDLINE]

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