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Nat Methods. 2010 Jul;7(7):519-20. doi: 10.1038/nmeth.1465. Epub 2010 May 30.

Estimating prion concentration in fluids and tissues by quantitative PMCA.

Author information

1
Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology, University of Texas Houston Medical School, Houston, Texas, USA.

Abstract

Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrP(Sc)) in brain, spleen, blood and urine of scrapie-affected hamsters.

PMID:
20512142
PMCID:
PMC4049222
DOI:
10.1038/nmeth.1465
[Indexed for MEDLINE]
Free PMC Article

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