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Ups J Med Sci. 2009;114(2):116-20. doi: 10.1080/03009730802688835.

Elevated levels of thymidine kinase 1 peptide in serum from patients with breast cancer.

Author information

1
Department of Medical Sciences, Clinical Chemistry, University Hospital, Uppsala, Sweden. Lena.carlsson@medsci.uu.se

Abstract

OBJECTIVES:

Thymidine kinase (TK) has an important role in DNA synthesis and is thus related to cell proliferation and turn-over. Traditionally, TK has been measured by enzymatic activity or radioimmunoassays. These assays are difficult to adapt to random access instruments. The aim of this study was to evaluate a new immunological sandwich assay for detection of TK peptides in serum from breast cancer patients.

MATERIAL AND METHODS:

Serum samples were collected from patients with breast cancer and stored frozen at -70 degrees C. The samples were collected after surgery, after metastatic tumor recurrence and after chemotherapy due to tumour recurrence. Patients' serum samples were analysed by the TK enzyme-linked immunosorbent assay (ELISA).

RESULTS:

In receiver operating characteristics (ROC) analyses of TK1 for diagnosis of breast cancer, the area under the curve (AUC) collected four weeks after surgery was 0.56 (95% CI 0.47-0.65), for samples collected postsurgically after tumour recurrence 0.73 (95% CI 0.65-0.80), and after chemotherapy 0.64 (95% CI 0.56-0.72).

CONCLUSIONS:

This study indicates that the tumour proliferation marker TK has a potential as a serum marker in breast cancer. Further studies are warranted to verify this observation.

PMID:
19396699
PMCID:
PMC2852757
DOI:
10.1080/03009730802688835
[Indexed for MEDLINE]
Free PMC Article

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