(a) AB1-rich domain area fraction plot as a function of AB1 weight fraction before (blue triangles) and after (red squares) NaOH treatment. The average AB1 area fractions are 60 ± 4% (av ± S.E., *N*_{vesicle} = 5, before NaOH) and 45 ± 2% (avg ± S.E., *N*_{vesicle} = 5, after NaOH) for an AB1 weight fraction of 60%, 48 ± 2% (av ± S.E., *N*_{vesicle} = 11, before NaOH) and 33 ± 4% (av ± S.E., *N*_{vesicle} = 19, after NaOH) for an AB1 weight fraction of 50%, and 22 ± 5% (av ± S.E., *N*_{vesicle} = 5, before NaOH) and 10 ± 1% (av ± S.E., *N*_{vesicle} = 5, after NaOH) for an AB1 weight fraction of 25%. Solid and dashed lines are linear fits to squares and triangles, respectively. The parallel trend of these two lines indicates that the changes in area fractions after NaOH treatment are roughly identical for different mixing ratios. Linear fits: before NaOH addition, *y* = (0.98 ± 0.06)*x, R*^{2} = 1.0; after NaOH addition, *y* = (1.08 ± 0.04)*x, R*^{2} = 1.0. (b) Effect of NaOH on individual domain circularity. After 24 h of incubation, domain circularity is reduced in a dose-dependent manner. Fit: *y* = 0.42 exp[−*x*/0.46] + 0.58, *N*_{domain} = 8.

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