Downregulation of miR-497-5p Improves Sepsis-Induced Acute Lung Injury by Targeting IL2RB

Biomed Res Int. 2021 Apr 12:2021:6624702. doi: 10.1155/2021/6624702. eCollection 2021.

Abstract

Introduction: Acute lung injury (ALI) induced by sepsis is a process related to inflammatory reactions, which involves lung cell apoptosis and production of inflammatory cytokine. Here, lipopolysaccharide (LPS) was applied to stimulate the mouse or human normal lung epithelial cell line (BEAS-2B) to construct a sepsis model in vivo and in vitro, and we also investigated the effect of miR-497-5p on sepsis-induced ALI. Material and Methods. Before LPS treatment, miR-497-5p antagomir was injected intravenously into mice to inhibit miR-497-5p expression in vivo. Similarly, miR-497-5p was knocked down in BEAS-2B cells. Luciferase reporter assay was applied to predict and confirm the miR-497-5p target gene. Cell viability, apoptosis, the levels of miR-497-5p, IL2RB, SP1, inflammatory cytokine, and lung injury were assessed.

Results: In BEAS-2B cells, a significant increase of apoptosis and inflammatory cytokine was shown after LPS stimulation. In septic mice, increased inflammatory cytokine production and apoptosis in lung cells and pulmonary morphological abnormalities were shown. The miR-497-5p inhibitor transfection showed antiapoptotic and anti-inflammatory effects on BEAS-2B cells upon LPS stimulation. In septic mice, the miR-497-5p antagomir injection also alleviated ALI, apoptosis, and inflammation caused by sepsis. The downregulation of IL2RB in BEAS-2B cells reversed the protective effects of the miR-497-5p inhibitor against ALI.

Conclusion: In conclusion, downregulation of miR-497-5p reduced ALI caused by sepsis through targeting IL2RB, indicating the potential effect of miR-497-5p for improving ALI caused by sepsis.

MeSH terms

  • Acute Lung Injury / etiology*
  • Animals
  • Apoptosis
  • Base Sequence
  • Cell Line
  • Cytokines / metabolism
  • Down-Regulation / genetics*
  • Humans
  • Inflammation Mediators / metabolism
  • Interleukin-2 Receptor beta Subunit / metabolism*
  • Lipopolysaccharides
  • Male
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Promoter Regions, Genetic / genetics
  • Sepsis / complications*
  • Sp1 Transcription Factor / metabolism
  • Up-Regulation / genetics

Substances

  • Cytokines
  • IL2RB protein, human
  • Il2rb protein, mouse
  • Inflammation Mediators
  • Interleukin-2 Receptor beta Subunit
  • Lipopolysaccharides
  • MIRN497 microRNA, human
  • MicroRNAs
  • Sp1 Transcription Factor
  • mirn497 microRNA, mouse