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Int Immunopharmacol. 2017 Sep;50:178-185. doi: 10.1016/j.intimp.2017.06.023. Epub 2017 Jun 28.

Direct effects of interleukin-8 on growth and functional activity of T lymphocytes.

Author information

1
Immanuel Kant Baltic Federal University, 14 A.Nevskogo St., Kaliningrad 236016, Russia; Russian Research Center of Medical Rehabilitation and Balneotherapy, 32 Novy Arbat St., Moscow 121099, Russia. Electronic address: max89me@yandex.ru.
2
Immanuel Kant Baltic Federal University, 14 A.Nevskogo St., Kaliningrad 236016, Russia. Electronic address: vvslon@rambler.ru.
3
Immanuel Kant Baltic Federal University, 14 A.Nevskogo St., Kaliningrad 236016, Russia. Electronic address: enant@list.ru.
4
Immanuel Kant Baltic Federal University, 14 A.Nevskogo St., Kaliningrad 236016, Russia. Electronic address: n_gazatova@mail.ru.
5
Immanuel Kant Baltic Federal University, 14 A.Nevskogo St., Kaliningrad 236016, Russia. Electronic address: omelashchenko@kantiana.ru.
6
Immanuel Kant Baltic Federal University, 14 A.Nevskogo St., Kaliningrad 236016, Russia. Electronic address: agoncharov59@mail.ru.
7
Scientific Research Institute of Clinical Immunology, Siberian Branch, Academy of Medical Sciences of Russia, 14 Yadrintsevskaya St., Novosibirsk 630099, Russia. Electronic address: galina-seledtsova@yandex.ru.
8
Immanuel Kant Baltic Federal University, 14 A.Nevskogo St., Kaliningrad 236016, Russia; Russian Research Center of Medical Rehabilitation and Balneotherapy, 32 Novy Arbat St., Moscow 121099, Russia. Electronic address: seledtsov@rambler.ru.

Abstract

CD3+ T-lymphocytes were isolated from the normal donors by positive magnetic separation. Activation of the T cells with particles conjugated with antibodies to CD3, СD28 and СD2 molecules led to a marked increase in T-cell production of interleukine-8 (IL-8). We present evidence that IL-8 receptor α-chain (CXCR1, CD181) is expressed on the cell surface of 13.3% T cells. Activation of T-lymphocytes resulted in significant enhancement of CD181+ cells both in naive CD4+ T cell and terminally differentiated effector CD4+ T cell compartments with concomitant reduction of CD181+ cells in effector memory CD4+ T cell subset. The level of T cell activation was assessed judging from the surface expression of CD25 (IL-2 receptor α-chain). We demonstrate that IL-8 treatment (0.01-10.0ng/ml concentration range) reduced the activation status of both CD4- and CD4+ effector memory T cells, as well as terminally differentiated effector T cells, without significantly affecting the activation of naive T cells or central memory T cells. In addition, IL-8 up-regulated IL-2 and down-regulated IL-10 production by activated T cells, with no effect on interferon-gamma (IFN-γ) and IL-4 production. Data obtained suggests the importance of IL-8 in the direct regulation of adaptive T cell reactivity.

KEYWORDS:

Adaptive immunity; CXCR1; Interleukin-8; T-cell subset

PMID:
28667886
DOI:
10.1016/j.intimp.2017.06.023
[Indexed for MEDLINE]

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