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Eur J Pharmacol. 2006 Mar 27;535(1-3):248-52. Epub 2006 Mar 9.

Differential effects of nitroblue tetrazolium on the hemodynamic responses elicited by activation of alpha1-adrenoceptors and 5-HT2 receptors in conscious rats.

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1
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-7389, USA.

Abstract

This study determined the effects of the lipophobic electron acceptor, nitroblue tetrazolium (5 micromol/kg, i.v.) on the vasoconstrictor responses elicited by the 5-hydroxytryptamine2 (5-HT2) receptor agonist, alpha-methyl-5-HT (5-50 microg/kg, i.v.) and the alpha1-adrenoceptor agonist, phenylephrine (2.5-20 microg/kg, i.v.) in conscious Sprague-Dawley rats. The systemic injection of nitroblue tetrazolium elicited pronounced hemodynamic responses that subsided by 10-15 min. Prior to the administration of nitroblue tetrazolium, the injections of alpha-methyl-5-HT and phenylephrine elicited dose-dependent increases in mean arterial blood pressure and mesenteric, renal and hindquarter vascular resistances. After administration of nitroblue tetrazolium, the vasoconstrictor responses elicited by alpha-methyl-5-HT were augmented whereas those elicited by phenylephrine were diminished. These results are consistent with the possibility that nitroblue tetrazolium interacts with the extracellular ligand-binding domains of 5-HT2 receptors and alpha1-adrenoceptor and that this interaction has opposite effects on activities of these G protein coupled receptors.

PMID:
16527267
DOI:
10.1016/j.ejphar.2006.01.060
[Indexed for MEDLINE]

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