Format

Send to

Choose Destination

See 1 citation found by title matching your search:

J Surg Res. 2013 Jun 1;182(1):101-7. doi: 10.1016/j.jss.2012.07.048. Epub 2012 Aug 8.

Development of a lethal, closed-abdomen grade V hepato-portal injury model in non-coagulopathic swine.

Author information

1
Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.

Abstract

BACKGROUND:

Hemorrhage within an intact abdominal cavity remains a leading cause of preventable death on the battlefield. Despite this need, there is no existing closed-cavity animal model to assess new hemostatic agents for the preoperative control of intra-abdominal hemorrhage.

METHODS:

We developed a novel, lethal liver injury model in non-coagulopathic swine by strategic placement of two wire loops in the medial liver lobes including the hepatic and portal veins. Distraction resulted in grade V liver laceration with hepato-portal injury, massive bleeding, and severe hypotension. Crystalloid resuscitation was started once mean arterial pressure (MAP) fell below 65 mm Hg. Monitoring continued for up to 180 min.

RESULTS:

We demonstrated 90% lethality (9/10) in swine receiving injury and fluid resuscitation, with a mean survival time of 43 min. Previous efforts in our laboratory to develop a consistently lethal swine model of abdominal solid organs, including preemptive anticoagulation, a two-hit injury with controlled hemorrhage prior to liver trauma, and the injury described above without resuscitation, consistently failed to result in lethal injury.

CONCLUSION:

This model can be used to screen other interventions for pre hospital control of noncompressible.

PMID:
22921917
DOI:
10.1016/j.jss.2012.07.048
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center