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Angew Chem Int Ed Engl. 2014 Jan 7;53(2):575-81. doi: 10.1002/anie.201305876. Epub 2013 Nov 29.

Detection of protein S-sulfhydration by a tag-switch technique.

Author information

1
Department of Chemistry, Washington State University, Pullman, WA 99164 (USA).

Abstract

Protein S-sulfhydration (forming -S-SH adducts from cysteine residues) is a newly defined oxidative posttranslational modification and plays an important role in H2 S-mediated signaling pathways. In this study we report the first selective, "tag-switch" method which can directly label protein S-sulfhydrated residues by forming stable thioether conjugates. Furthermore we demonstrate that H2 S alone cannot lead to S-sulfhydration and that the two possible physiological mechanisms include reaction with protein sulfenic acids (P-SOH) or the involvement of metal centers which would facilitate the oxidation of H2 S to HS(.) .

KEYWORDS:

hydrogen sulfide; protein S-sulfhydration; signal transduction; tag-switch; thiols

PMID:
24288186
PMCID:
PMC4306352
DOI:
10.1002/anie.201305876
[Indexed for MEDLINE]
Free PMC Article

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