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Nucleic Acids Res. 2010 Sep;38(17):5919-28. doi: 10.1093/nar/gkq342. Epub 2010 May 13.

Deep sequencing reveals differential expression of microRNAs in favorable versus unfavorable neuroblastoma.

Author information

1
University Children's Hospital Essen, Hufelandstr. 55, 45122 Essen, Germany. johannes.schulte@uni-due.de

Abstract

Small non-coding RNAs, in particular microRNAs(miRNAs), regulate fine-tuning of gene expression and can act as oncogenes or tumor suppressor genes. Differential miRNA expression has been reported to be of functional relevance for tumor biology. Using next-generation sequencing, the unbiased and absolute quantification of the small RNA transcriptome is now feasible. Neuroblastoma(NB) is an embryonal tumor with highly variable clinical course. We analyzed the small RNA transcriptomes of five favorable and five unfavorable NBs using SOLiD next-generation sequencing, generating a total of >188 000 000 reads. MiRNA expression profiles obtained by deep sequencing correlated well with real-time PCR data. Cluster analysis differentiated between favorable and unfavorable NBs, and the miRNA transcriptomes of these two groups were significantly different. Oncogenic miRNAs of the miR17-92 cluster and the miR-181 family were overexpressed in unfavorable NBs. In contrast, the putative tumor suppressive microRNAs, miR-542-5p and miR-628, were expressed in favorable NBs and virtually absent in unfavorable NBs. In-depth sequence analysis revealed extensive post-transcriptional miRNA editing. Of 13 identified novel miRNAs, three were further analyzed, and expression could be confirmed in a cohort of 70 NBs.

PMID:
20466808
PMCID:
PMC2943620
DOI:
10.1093/nar/gkq342
[Indexed for MEDLINE]
Free PMC Article

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