Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My NCBI Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1999 1
2000 1
2001 2
2003 8
2004 2
2005 14
2006 16
2007 13
2008 18
2009 9
2010 14
2011 16
2012 25
2013 23
2014 28
2015 19
2016 16
2017 22
2018 25
2019 21
2020 22
2021 21
2022 24
2023 13
2024 7

Text availability

Article attribute

Article type

Publication date

Search Results

337 results

Results by year

Filters applied: . Clear all
Page 1
Bone Formation in Zebrafish: The Significance of DAF-FM DA Staining for Nitric Oxide Detection.
Huysseune A, Larsen UG, Larionova D, Matthiesen CL, Petersen SV, Muller M, Witten PE. Huysseune A, et al. Biomolecules. 2023 Dec 12;13(12):1780. doi: 10.3390/biom13121780. Biomolecules. 2023. PMID: 38136650 Free PMC article.
DAF-FM DA is widely used as a live staining compound to show the presence of nitric oxide (NO) in cells. ...Von Kossa and Alizarin red S staining to reveal mineral deposits showed that DAF-FM DA stains both the mineralized and non-mineralized bone matr
DAF-FM DA is widely used as a live staining compound to show the presence of nitric oxide (NO) in cells. ...Von Kossa and Aliz
Kinetic analysis of DAF-FM activation by NO: toward calibration of a NO-sensitive fluorescent dye.
Namin SM, Nofallah S, Joshi MS, Kavallieratos K, Tsoukias NM. Namin SM, et al. Nitric Oxide. 2013 Jan 15;28:39-46. doi: 10.1016/j.niox.2012.10.001. Epub 2012 Oct 11. Nitric Oxide. 2013. PMID: 23063986 Free PMC article.
In this study we utilize a combination of mathematical modeling and experimentation to elucidate the kinetics of NO/DAF-FM reaction in solution. Modeling and experiments suggest that the slope of fluorescent intensity (FI) can be related to NO concentration accordin …
In this study we utilize a combination of mathematical modeling and experimentation to elucidate the kinetics of NO/DAF-FM rea …
Intracellular production of reactive oxygen species and a DAF-FM-related compound in Aspergillus fumigatus in response to antifungal agent exposure.
Oiki S, Nasuno R, Urayama SI, Takagi H, Hagiwara D. Oiki S, et al. Sci Rep. 2022 Aug 6;12(1):13516. doi: 10.1038/s41598-022-17462-y. Sci Rep. 2022. PMID: 35933435 Free PMC article.
The production of nitric oxide (NO) was determined using diaminofluorescein-FM diacetate (DAF-FM DA) and occurred in response to antifungal compounds and stress conditions. ...However, NO production was not detected in the hyphae using the Greiss method. An LC/MS an …
The production of nitric oxide (NO) was determined using diaminofluorescein-FM diacetate (DAF-FM DA) and occurred in response …
Detection of Nitric Oxide from Chickpea Using DAF Fluorescence and Chemiluminescence Methods.
Kumari A, Bhatoee M, Singh P, Kaladhar VC, Yadav N, Paul D, Loake GJ, Gupta KJ. Kumari A, et al. Curr Protoc. 2022 Apr;2(4):e420. doi: 10.1002/cpz1.420. Curr Protoc. 2022. PMID: 35441832
In this article, we describe a detailed procedure for the measurement of NO by diaminofluorescein (DAF) in cell-permeable forms (DAF-FM-DA). In this method, the tissues are immersed in DAF-FM DA, leading to their diffusion from the plasma membrane to t …
In this article, we describe a detailed procedure for the measurement of NO by diaminofluorescein (DAF) in cell-permeable forms (DAF- …
DAF-FM (4-amino-5-methylamino-2',7'-difluorofluorescein) diacetate detects impairment of agonist-stimulated nitric oxide synthesis by elevated glucose in human vascular endothelial cells: reversal by vitamin C and L-sepiapterin.
Sheng JZ, Wang D, Braun AP. Sheng JZ, et al. J Pharmacol Exp Ther. 2005 Nov;315(2):931-40. doi: 10.1124/jpet.105.087932. Epub 2005 Aug 10. J Pharmacol Exp Ther. 2005. PMID: 16093274
In the present study, we have utilized a recently developed NO-sensitive fluorescent dye, DAF-FM (4-amino-5-methylamino-2',7'-difluorofluorescein) diacetate to directly examine the consequences of elevated glucose on agonist-evoked NO synthesis in cultured human vas …
In the present study, we have utilized a recently developed NO-sensitive fluorescent dye, DAF-FM (4-amino-5-methylamino-2',7'- …
Procalcitonin mediates vascular dysfunction in obesity.
Brabenec L, Hellenthal KEM, Müller M, Kintrup S, Zurek-Leffers F, Kardell M, Otto M, Wagner NM. Brabenec L, et al. Life Sci. 2022 Oct 15;307:120889. doi: 10.1016/j.lfs.2022.120889. Epub 2022 Aug 20. Life Sci. 2022. PMID: 35998685
Murine endothelial cells were isolated using CD31-antibody-coated magnetic beads and reactive oxygen species and nitric oxide (NO) determined by H2DCF- or DAF-FM diacetate loading. Endothelium-dependent vasorelaxation was analyzed using pressure myography of murine …
Murine endothelial cells were isolated using CD31-antibody-coated magnetic beads and reactive oxygen species and nitric oxide (NO) determine …
NO signaling in retinal bipolar cells.
Agurto A, Vielma AH, Cadiz B, Couve E, Schmachtenberg O. Agurto A, et al. Exp Eye Res. 2017 Aug;161:30-35. doi: 10.1016/j.exer.2017.05.013. Epub 2017 Jun 1. Exp Eye Res. 2017. PMID: 28579034
This study correlates NO synthesis in dark-adapted retinas, through labeling with the NO marker DAF-FM, with neuronal nitric oxide synthase (nNOS) and inducible NOS expression, and presence of the NO receptor soluble guanylate cyclase in bipolar cells. ...
This study correlates NO synthesis in dark-adapted retinas, through labeling with the NO marker DAF-FM, with neuronal nitric o …
Methods of nitric oxide detection in plants: a commentary.
Mur LA, Mandon J, Cristescu SM, Harren FJ, Prats E. Mur LA, et al. Plant Sci. 2011 Nov;181(5):509-19. doi: 10.1016/j.plantsci.2011.04.003. Epub 2011 Apr 13. Plant Sci. 2011. PMID: 21893246 Review.
This arises from a series of causes: (a) doubts have arisen over the specificity of widely used 4,5-diaminofluorescein diacetate (DAF-2DA)/4-amino-5-methylamino-2,7-difluorofluorescein (DAF-FM) dyes for NO, (b) no plant nitric oxide synthase (NOS) has been cloned, s …
This arises from a series of causes: (a) doubts have arisen over the specificity of widely used 4,5-diaminofluorescein diacetate (DAF-2DA)/4 …
Nitric oxide production by glomerular podocytes.
Palygin O, Ilatovskaya DV, Levchenko V, Endres BT, Geurts AM, Staruschenko A. Palygin O, et al. Nitric Oxide. 2018 Jan 30;72:24-31. doi: 10.1016/j.niox.2017.11.005. Epub 2017 Nov 8. Nitric Oxide. 2018. PMID: 29128399 Free PMC article.
To test this, we isolated glomeruli from the kidneys of Dahl salt-sensitive (SS) rats fed a low salt (LS; 0.4% NaCl) or high salt (HS; 4% NaCl, 3 weeks) diets and loaded podocytes with either a combination of NO and Ca(2+) fluorophores (DAF-FM and Fura Red, respecti …
To test this, we isolated glomeruli from the kidneys of Dahl salt-sensitive (SS) rats fed a low salt (LS; 0.4% NaCl) or high salt (HS; 4% Na …
337 results