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  • Showing results for Cytotoxicity[Title] AND chlorhexidine[Title] AND human[Title] AND osteoblastic[Title] AND cells[Title] AND related[Title] AND intracellular[Title] AND levels[Title]. Your search for Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathoine levels retrieved no results.
Int Endod J. 2010 May;43(5):430-5. doi: 10.1111/j.1365-2591.2010.01700.x.

Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels.

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Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.



To evaluate the mechanisms of cytotoxicity of chlorhexidine (CHX) in human osteoblastic cells in vitro.


Cytotoxicity, cell proliferation and collagen synthesis assays were performed to elucidate the toxic effects of CHX on the human osteoblastic cell line U2OS. To determine whether glutathione (GSH) levels were important in the cytotoxicity of CHX, cells were pre-treated with 2-oxothiazolidine-4-carboxylic acid (OTZ) to boost GSH levels or buthionine sulfoximine (BSO) to deplete GSH.


CHX demonstrated a cytotoxic effect to U2OS cells in a dose-dependent manner (P < 0.05). The 50% inhibition concentration of CHX was approximately 0.005%. CHX also inhibited cell proliferation and collagen synthesis (P < 0.05). The addition of OTZ acted as a protective effect on the CHX-induced cytotoxicity (P < 0.05). In contrast, the addition of BSO enhanced the CHX-induced cytotoxicity (P < 0.05).


The levels of CHX tested inhibited cell growth, proliferation and collagen synthesis on U2OS cells. CHX has significant potential for periapical toxicity. GSH depletion might be one of the mechanisms underlying CHX cytotoxicity.

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