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Clin J Sport Med. 2012 Mar;22(2):132-9. doi: 10.1097/JSM.0b013e31823f310a.

Correlation between seminal oxidative stress biomarkers and antioxidants with sperm DNA damage in elite athletes and recreationally active men.

Author information

1
Department of Exercise Physiology, Faculty of Physical Education and Sport Science, Urmia University, Urmia, Iran.

Abstract

OBJECTIVE:

To evaluate the seminal plasma 8-isoprostane, reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), catalase, total antioxidant capacity (TAC), and levels of sperm DNA fragmentation in elite athletes and recreationally active men.

DESIGN:

Prospective design was used for this study.

SETTING:

The study was performed in the Exercise Physiology Laboratory of the Urmia University.

PARTICIPANTS:

Fifty-six elite athletes and 52 recreationally active men (18-28 years) participated in this study.

INTERVENTION:

All subjects had a semen sampling at baseline.

MAIN OUTCOME MEASURES:

Total antioxidant capacity and SOD activity were measured by colorimetric assay. Levels of ROS were measured by a chemiluminescence assay. Malondialdehyde levels were measured by thiobarbituric acid reactive substance assay. Catalase activity was measured by monitoring the initial rate of disappearance of hydrogen peroxide. Concentration of free 8-isoprostane was measured by enzyme immunoassay method. Sperm DNA fragmentation was evaluated with the terminal deoxynucleotidyl transferase-mediated fluorescein dUTP nick end-labeling assay.

RESULTS:

Recreationally active men have significantly higher levels of body fat, seminal SOD, TAC, and catalase and lower levels of V[Combining Dot Above]O2max, seminal ROS, MDA, and 8-isoprostane and subsequently lower rate of sperm DNA fragmentation when compared with elite athletes (P < 0.001). Significantly negative correlation was observed between sperm DNA fragmentation with body fat, seminal SOD, catalase, and TAC levels (P < 0.001). Significantly positive correlation was observed between sperm DNA fragmentation with V[Combining Dot Above]O2max, seminal 8-isoprostane, ROS, and MDA levels (P < 0.001).

CONCLUSION:

Spermatozoa from recreationally active men may be less susceptible to oxidative stress-induced DNA damage and hence infertility.

PMID:
22246344
DOI:
10.1097/JSM.0b013e31823f310a
[Indexed for MEDLINE]

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