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Trans R Soc Trop Med Hyg. 2013 Apr;107(4):269-71. doi: 10.1093/trstmh/trs094. Epub 2013 Feb 20.

Comparison of real-time PCR and Kato smear microscopy for the detection of hookworm infections in three consecutive faecal samples from schoolchildren in Ghana.

Author information

1
Department of Medical Microbiology, University Medical Centre, Utrecht, The Netherlands.

Abstract

BACKGROUND:

The classic technique used to detect hookworm infections in population-based surveys is microscopic examination of Kato thick smears of multiple faecal samples per person as variation in soil-transmitted helminth egg output is common.

METHODS:

As an alternative to this time-consuming and logistically difficult procedure, a PCR-based method to detect hookworm infections was evaluated. Faecal samples collected from 65 Ghanaian school children during February-June 2006 were examined using both techniques.

RESULTS:

Thirty-one children with a hookworm infection were detected by Kato examination of three faecal samples compared with 30 children detected by PCR of a single faecal sample and 39 detected by PCR of three faecal samples.

CONCLUSION:

PCR provides a sensitive alternative to the conventional microscopic detection of hookworm infections.

PMID:
23426113
DOI:
10.1093/trstmh/trs094
[Indexed for MEDLINE]

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