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J Wound Care. 2014 Oct;23(10):465-74, 476. doi: 10.12968/jowc.2014.23.10.465.

Comparison of cryopreserved amniotic membrane and umbilical cord tissue with dehydrated amniotic membrane/chorion tissue.

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1
Amniox Medical, Atlanta, GA, 30339, USA;

Abstract

OBJECTIVE:

To evaluate how the different processing methods cryopreservation and dehydration affect the structural integrity and biological composition of key signalling molecules within amniotic membrane and umbilical cord tissues.

METHOD:

We directly compared cryopreserved amniotic membrane (AM) and umbilical cord (UC) tissues with dehydrated amniotic membrane/chorion (dHACM) tissue using biochemical and functional assays including histological and histochemical staining, BCA, agarose gel electrophoresis, western blot, ELISA, and proliferation and cell death assays.

RESULTS:

Cryopreservation retains the native architecture of the AM/UC extracellular matrix and maintains the quantity and activity of key biological signals present in fresh AM/UC, including high molecular weight hyaluronic acid, heavy chain-HA complex, and pentraxin 3. In contrast, dehydrated tissues were structurally compromised and almost completely lacked these crucial components.

CONCLUSION:

The results presented here indicate that cryopreservation better preserves the structural and biological signaling molecules of foetal tissues.

KEYWORDS:

amniotic membrane; anti-inflammatory; anti-scarring; cryopreserved; dehydrated; umbilical cord

PMID:
25296347
DOI:
10.12968/jowc.2014.23.10.465
[Indexed for MEDLINE]

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