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Cancer Gene Ther. 2015 Jul;22(7):344-50. doi: 10.1038/cgt.2015.26. Epub 2015 Jun 19.

Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery.

Author information

1
1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA [3] Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
2
1] AntiCancer, San Diego, CA, USA [2] Department of Surgery, University of California San Diego, San Diego, CA, USA.
3
Department of Surgery, University of California San Diego, San Diego, CA, USA.
4
Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
5
Department of Gastroenterology, Gifu University Graduate School of Medicine, Gifu, Japan.
6
Department of Surgical Oncoloy, MD Anderson Cancer Center, University of Texas, Houston, TX, USA.
7
Oncolys BioPharm, Tokyo, Japan.
8
Center for Innovative Clinical Medicine, Okayama University Hospital, Okayama, Japan.

Abstract

Precise fluorescence-guided surgery (FGS) for pancreatic cancer has the potential to greatly improve the outcome in this recalcitrant disease. To achieve this goal, we have used genetic reporters to color code cancer and stroma cells in a patient-derived orthotopic xenograft (PDOX) model. The telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 was used to label the cancer cells of a pancreatic cancer PDOX. The PDOX was previously grown in a red fluorescent protein (RFP) transgenic mouse that stably labeled the PDOX stroma cells bright red. The color-coded PDOX model enabled FGS to completely resect the pancreatic tumors including stroma. Dual-colored FGS significantly prevented local recurrence, which bright-light surgery or single-color FGS could not. FGS, with color-coded cancer and stroma cells has important potential for improving the outcome of recalcitrant-cancer surgery.

PMID:
26088297
PMCID:
PMC4523223
DOI:
10.1038/cgt.2015.26
[Indexed for MEDLINE]
Free PMC Article

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