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Jack bean urease (EC 3.5.1.5). IV. The molecular size and the mechanism of inhibition by hydroxamic acids. Spectrophotometric titration of enzymes with reversible inhibitors.
Dixon NE, Hinds JA, Fihelly AK, Gazzola C, Winzor DJ, Blakeley RL, Zerner B. Dixon NE, et al. Can J Biochem. 1980 Dec;58(12):1323-34. doi: 10.1139/o80-180. Can J Biochem. 1980. PMID: 7248834
Consistent values for the equivalent weight are obtained by a routine spectrophotometric titration of the active site of freshly prepared urease with trans-cinnamoylhydroxamic acid. General equations are derived which describe spectrophotometric titrations of bindin …
Consistent values for the equivalent weight are obtained by a routine spectrophotometric titration of the active site of freshly prepared ur …