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Int J Mol Sci. 2017 Feb 9;18(2). pii: E369. doi: 10.3390/ijms18020369.

Chitosan-Recombinamer Layer-by-Layer Coatings for Multifunctional Implants.

Author information

1
Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN 55108, USA. gprasaad88@gmail.com.
2
Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA. chenx838@umn.edu.
3
Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA. lixx1191@umn.edu.
4
BIOFORGE Group, University of Valladolid, Valladolid 4701, Spain. roca@bioforge.uva.es.
5
Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN 55108, USA. bhatt002@umn.edu.
6
Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA. apari003@umn.edu.

Abstract

The main clinical problems for dental implants are (1) formation of biofilm around the implant-a condition known as peri-implantitis and (2) inadequate bone formation around the implant-lack of osseointegration. Therefore, developing an implant to overcome these problems is of significant interest to the dental community. Chitosan has been reported to have good biocompatibility and anti-bacterial activity. An osseo-inductive recombinant elastin-like biopolymer (P-HAP), that contains a peptide derived from the protein statherin, has been reported to induce biomineralization and osteoblast differentiation. In this study, chitosan/P-HAP bi-layers were built on a titanium surface using a layer-by-layer (LbL) assembly technique. The difference in the water contact angle between consecutive layers, the representative peaks in diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), X-ray photoelectron spectroscopy (XPS), and the changes in the topography between surfaces with a different number of bi-layers observed using atomic force microscopy (AFM), all indicated the successful establishment of chitosan/P-HAP LbL assembly on the titanium surface. The LbL-modified surfaces showed increased biomineralization, an appropriate mouse pre-osteoblastic cell response, and significant anti-bacterial activity against Streptococcus gordonii, a primary colonizer of tissues in the oral environment.

KEYWORDS:

chitosan; elastin-like recombinamers; implant; layer-by-layer; titanium

PMID:
28208793
PMCID:
PMC5343904
DOI:
10.3390/ijms18020369
[Indexed for MEDLINE]
Free PMC Article

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