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Biol Cell. 2019 Apr;111(4):95-107. doi: 10.1111/boc.201800042. Epub 2019 Feb 14.

Cell type-specific structural plasticity of the ciliary transition zone in C. elegans.

Author information

1
Department of Genetics and Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ, 08854, USA.
2
Department of Biology, University of Utah, Salt Lake City, UT, 84112, USA.
3
Center for C. elegans Anatomy, Albert Einstein College of Medicine, Bronx, NY, 10461, USA.
4
Simons Electron Microscopy Center, New York Structural Biology Center, NY, 10027, USA.

Abstract

BACKGROUND INFORMATION:

The current consensus on cilia development posits that the ciliary transition zone (TZ) is formed via extension of nine centrosomal microtubules. In this model, TZ structure remains unchanged in microtubule number throughout the cilium life cycle. This model does not however explain structural variations of TZ structure seen in nature and could also lend itself to the misinterpretation that deviations from nine-doublet microtubule ultrastructure represent an abnormal phenotype. Thus, a better understanding of events that occur at the TZ in vivo during metazoan development is required.

RESULTS:

To address this issue, we characterized ultrastructure of two types of sensory cilia in developing Caenorhabditis elegans. We discovered that, in cephalic male (CEM) and inner labial quadrant (IL2Q) sensory neurons, ciliary TZs are structurally plastic and remodel from one structure to another during animal development. The number of microtubule doublets forming the TZ can be increased or decreased over time, depending on cilia type. Both cases result in structural TZ intermediates different from TZ in cilia of adult animals. In CEM cilia, axonemal extension and maturation occurs concurrently with TZ structural maturation.

CONCLUSIONS AND SIGNIFICANCE:

Our work extends the current model to include the structural plasticity of metazoan transition zone, which can be structurally delayed, maintained or remodelled in cell type-specific manner.

KEYWORDS:

C. elegans; axoneme; cilia; microtubule; transition zone

PMID:
30681171
DOI:
10.1111/boc.201800042
[Indexed for MEDLINE]

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