Format

Send to

Choose Destination

See 1 citation found by title matching your search:

Traffic. 2018 Mar;19(3):166-181. doi: 10.1111/tra.12544. Epub 2018 Feb 12.

Cargo crowding at actin-rich regions along axons causes local traffic jams.

Author information

1
Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India.
2
Neurobiology, National Centre for Biological Sciences, Bangalore, India.
3
The Institute of Mathematical Sciences, CIT Campus, Chennai, India.
4
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri.
5
Homi Bhabha National Institute, Training School Complex, Mumbai, India.

Abstract

Steady axonal cargo flow is central to the functioning of healthy neurons. However, a substantial fraction of cargo in axons remains stationary up to several minutes. We examine the transport of precursors of synaptic vesicles (pre-SVs), endosomes and mitochondria in Caenorhabditis elegans touch receptor neurons, showing that stationary cargo are predominantly present at actin-rich regions along the neuronal process. Stationary vesicles at actin-rich regions increase the propensity of moving vesicles to stall at the same location, resulting in traffic jams arising from physical crowding. Such local traffic jams at actin-rich regions are likely to be a general feature of axonal transport since they also occur in Drosophila neurons. Repeated touch stimulation of C. elegans reduces the density of stationary pre-SVs, indicating that these traffic jams can act as both sources and sinks of vesicles. This suggests that vesicles trapped in actin-rich regions are functional reservoirs that may contribute to maintaining robust cargo flow in the neuron. A video abstract of this article can be found at: Video S1; Video S2.

KEYWORDS:

Caenorhabditis elegans; Drosophila melanogaster; actin; axonal transport; neuronal stimulation; physical crowding; precursors of synaptic vesicles; reservoirs; stationary cargo; stationary vesicles; touch receptor neurons; traffic jams

PMID:
29178177
DOI:
10.1111/tra.12544
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center