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Items: 1 to 20 of 62

1.

Using CAPTURE to detect spacer acquisition in native CRISPR arrays.

McKenzie RE, Almendros C, Vink JNA, Brouns SJJ.

Nat Protoc. 2019 Feb 11. doi: 10.1038/s41596-018-0123-5. [Epub ahead of print]

PMID:
30742049
2.

Molecular and Evolutionary Determinants of Bacteriophage Host Range.

de Jonge PA, Nobrega FL, Brouns SJJ, Dutilh BE.

Trends Microbiol. 2019 Jan;27(1):51-63. doi: 10.1016/j.tim.2018.08.006. Epub 2018 Sep 1. Review.

PMID:
30181062
3.

Role of nucleotide identity in effective CRISPR target escape mutations.

Künne T, Zhu Y, da Silva F, Konstantinides N, McKenzie RE, Jackson RN, Brouns SJ.

Nucleic Acids Res. 2018 Nov 2;46(19):10395-10404. doi: 10.1093/nar/gky687.

4.

Targeting mechanisms of tailed bacteriophages.

Nobrega FL, Vlot M, de Jonge PA, Dreesens LL, Beaumont HJE, Lavigne R, Dutilh BE, Brouns SJJ.

Nat Rev Microbiol. 2018 Dec;16(12):760-773. doi: 10.1038/s41579-018-0070-8. Review.

PMID:
30104690
5.

Complete Genome Sequences of Two T4-Like Escherichia coli Bacteriophages.

Costa AR, Brouns SJJ, Nobrega FL.

Genome Announc. 2018 Jun 28;6(26). pii: e00586-18. doi: 10.1128/genomeA.00586-18.

6.

Repetitive DNA Reeling by the Cascade-Cas3 Complex in Nucleotide Unwinding Steps.

Loeff L, Brouns SJJ, Joo C.

Mol Cell. 2018 May 3;70(3):385-394.e3. doi: 10.1016/j.molcel.2018.03.031. Epub 2018 Apr 26.

PMID:
29706536
7.

Cas4 Facilitates PAM-Compatible Spacer Selection during CRISPR Adaptation.

Kieper SN, Almendros C, Behler J, McKenzie RE, Nobrega FL, Haagsma AC, Vink JNA, Hess WR, Brouns SJJ.

Cell Rep. 2018 Mar 27;22(13):3377-3384. doi: 10.1016/j.celrep.2018.02.103.

8.

Complete Genome Sequence of the Escherichia coli Phage Ayreon.

Vlot M, Nobrega FL, Wong CFA, Liu Y, Brouns SJJ.

Genome Announc. 2018 Jan 11;6(2). pii: e01354-17. doi: 10.1128/genomeA.01354-17.

9.

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR-Cas effector complexes.

Vlot M, Houkes J, Lochs SJA, Swarts DC, Zheng P, Kunne T, Mohanraju P, Anders C, Jinek M, van der Oost J, Dickman MJ, Brouns SJJ.

Nucleic Acids Res. 2018 Jan 25;46(2):873-885. doi: 10.1093/nar/gkx1264.

10.

CRISPR-Cas: Adapting to change.

Jackson SA, McKenzie RE, Fagerlund RD, Kieper SN, Fineran PC, Brouns SJ.

Science. 2017 Apr 7;356(6333). pii: eaal5056. doi: 10.1126/science.aal5056. Epub 2017 Apr 6. Review.

PMID:
28385959
11.

Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR-Cas system.

Staals RH, Jackson SA, Biswas A, Brouns SJ, Brown CM, Fineran PC.

Nat Commun. 2016 Oct 3;7:12853. doi: 10.1038/ncomms12853.

12.

Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation.

Künne T, Kieper SN, Bannenberg JW, Vogel AI, Miellet WR, Klein M, Depken M, Suarez-Diez M, Brouns SJ.

Mol Cell. 2016 Sep 1;63(5):852-64. doi: 10.1016/j.molcel.2016.07.011. Epub 2016 Aug 18.

13.

CRISPR interference and priming varies with individual spacer sequences.

Xue C, Seetharam AS, Musharova O, Severinov K, Brouns SJ, Severin AJ, Sashital DG.

Nucleic Acids Res. 2015 Dec 15;43(22):10831-47. doi: 10.1093/nar/gkv1259. Epub 2015 Nov 19.

14.

CRISPR sabotage.

van der Oost J, Brouns SJ.

Genome Biol. 2015 Nov 9;16:248. doi: 10.1186/s13059-015-0820-0.

15.

An updated evolutionary classification of CRISPR-Cas systems.

Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM, Terns MP, White MF, Yakunin AF, Garrett RA, van der Oost J, Backofen R, Koonin EV.

Nat Rev Microbiol. 2015 Nov;13(11):722-36. doi: 10.1038/nrmicro3569. Epub 2015 Sep 28. Review.

16.

Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes.

Künne T, Westra ER, Brouns SJ.

Methods Mol Biol. 2015;1311:171-84. doi: 10.1007/978-1-4939-2687-9_11.

PMID:
25981473
17.

Two distinct DNA binding modes guide dual roles of a CRISPR-Cas protein complex.

Blosser TR, Loeff L, Westra ER, Vlot M, Künne T, Sobota M, Dekker C, Brouns SJJ, Joo C.

Mol Cell. 2015 Apr 2;58(1):60-70. doi: 10.1016/j.molcel.2015.01.028. Epub 2015 Mar 5.

18.

Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli.

Jackson RN, Golden SM, van Erp PB, Carter J, Westra ER, Brouns SJ, van der Oost J, Terwilliger TC, Read RJ, Wiedenheft B.

Science. 2014 Sep 19;345(6203):1473-9. doi: 10.1126/science.1256328. Epub 2014 Aug 7.

19.

Archaeal MBF1 binds to 30S and 70S ribosomes via its helix-turn-helix domain.

Blombach F, Launay H, Snijders AP, Zorraquino V, Wu H, de Koning B, Brouns SJ, Ettema TJ, Camilloni C, Cavalli A, Vendruscolo M, Dickman MJ, Cabrita LD, La Teana A, Benelli D, Londei P, Christodoulou J, van der Oost J.

Biochem J. 2014 Sep 1;462(2):373-84. doi: 10.1042/BJ20131474.

PMID:
24825021
20.

Degenerate target sites mediate rapid primed CRISPR adaptation.

Fineran PC, Gerritzen MJ, Suárez-Diez M, Künne T, Boekhorst J, van Hijum SA, Staals RH, Brouns SJ.

Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):E1629-38. doi: 10.1073/pnas.1400071111. Epub 2014 Apr 7.

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