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Chin J Integr Med. 2014 Mar 2. [Epub ahead of print]

Bioactivity-guided identification to delineate the immunomodulatory effects of methanolic extract of Nigella sativa seed on human peripheral blood mononuclear cells.

Author information

1
Molecular Cancer Biology Research Lab, Department of Food Science and Nutrition, King Saud University, Riyadh, 11451, Saudi Arabia, alialshatwi@gmail.com.

Abstract

OBJECTIVE:

To study the immunomodulatory effects of Nigella sativa (NS) on human peripheral blood mononuclear cells (PBMCs) on a Phytohemagglutinin (PHA) and a non-PHA stimulated proliferation.

METHODS:

Cells isolated from human PBMCs which were treated with methanolic extract of NS for 48 h into two separate environments (PHA and non-PHA stimulated). Flow cytometry (for T helper/inducer cells and natural killer cells) and real time-polymerase chain reaction (PCR) assays for a few selected proinflammatory gene expressions were performed.

RESULTS:

Extracts from NS had an immunostimulating effect on non-PHA-stimulated proliferation of human PBMCs. In contrast, immunosuppressive activity was observed on PHA-stimulated proliferation of human PBMCs.

CONCLUSION:

This in vitro study revealed the effects of NS plant extract on nonspecifific cellular immune responses.

PMID:
24584754
DOI:
10.1007/s11655-013-1534-3

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