Format

Send to

Choose Destination

See 1 citation found by title matching your search:

Sci Adv. 2018 Aug 1;4(8):eaat5107. doi: 10.1126/sciadv.aat5107. eCollection 2018 Aug.

BioBits™ Bright: A fluorescent synthetic biology education kit.

Author information

1
Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Technological Institute E136, Evanston, IL 60208-3120, USA.
2
Chemistry of Life Processes Institute, Northwestern University, 2170 Campus Drive, Evanston, IL 60208-3120, USA.
3
Center for Synthetic Biology, Northwestern University, 2145 Sheridan Road, Technological Institute E136, Evanston, IL 60208-3120, USA.
4
Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.
5
Institute for Medical Engineering and Science, MIT, Cambridge, MA 02139, USA.
6
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
7
School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
8
Department of Biological Sciences, Northwestern University, 2205 Tech Drive, Hogan Hall 2144, Evanston, IL 60208, USA.
9
Department of Mechanical Engineering, Northwestern University, 2145 Sheridan Road, Technological Institute B224, Evanston, IL 60208-3120, USA.
10
Glenbard East High School, 1014 South Main Street, Lombard, IL 60148-3938, USA.
11
Amos Alonzo Stagg High School, 8015 West 111th Street, Palos Hills, IL 60465-2203, USA.
12
Grover Cleveland Elementary School, 3121 West Byron Street, Chicago, IL 60618-3403, USA.
13
Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA.
14
Glenbrook South High School, 4000 West Lake Avenue, Glenview, IL 60026-1239, USA.
15
Jones College Prep High School, 700 South State Street, Chicago, IL 60605-2109, USA.
16
Gwendolyn Brooks College Preparatory Academy, 250 East 111th Street, Chicago, IL 60628-4324, USA.
17
Delta High School, 3400 East SR 28, Muncie, IN 47303, USA.
18
ASPIRA Business and Finance High School, 2989 North Milwaukee Avenue, Chicago, IL 60618-7347, USA.
19
Aptakisic-Tripp School District 102, 850 Highland Grove Drive, Buffalo Grove, IL 60089, USA.
20
Oak Lawn Hometown Middle School, 5345 West 99th Street, Oak Lawn, IL 60453-3815, USA.
21
Hoffman Estates High School, 1100 West Higgins Road, Hoffman Estates, IL 60169-4050, USA.
22
Vernon Hills High School, 145 Lakeview Parkway, Vernon Hills, IL 60061-1566, USA.
23
Morgan Park High School, 1744 West Pryor Avenue, Chicago, IL 60643-3497, USA.
24
Northside College Prep High School, 5501 North Kedzie Avenue, Chicago, IL 60625-3923, USA.
25
Aqsa School, 7361 West 92nd Street, Bridgeview, IL 60455-2133, USA.
26
Adlai E. Stevenson High School, 1 Stevenson Drive, Lincolnshire, IL 60069-2824, USA.
27
Lyons Township High School, 100 South Brainard Avenue, La Grange, IL 60525-2101, USA.
28
Leslie Dan Faculty of Pharmacy, University of Toronto, 144 College Street, Toronto, Ontario M5S 3M2, Canada.
29
Synthetic Biology Center, MIT, Cambridge, MA 02139, USA.
30
Harvard-MIT Program in Health Sciences and Technology, Cambridge, MA 02139, USA.
31
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
32
Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 676 North Saint Clair Street, Suite 1200, Chicago, IL 60611-3068, USA.
33
Simpson Querrey Institute, Northwestern University, 303 East Superior Street, Suite 11-131, Chicago, IL 60611-2875, USA.

Abstract

Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However, implementation of hands-on biology activities in classrooms is challenging because of the need for specialized equipment and expertise to grow living cells. We present BioBits™ Bright, a shelf-stable, just-add-water synthetic biology education kit with easy visual outputs enabled by expression of fluorescent proteins in freeze-dried, cell-free reactions. We introduce activities and supporting curricula for teaching the central dogma, tunable protein expression, and design-build-test cycles and report data generated by K-12 teachers and students. We also develop inexpensive incubators and imagers, resulting in a comprehensive kit costing <US$100 per 30-person classroom. The user-friendly resources of this kit promise to enhance biology education both inside and outside the classroom.

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center