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Int J Biol Macromol. 2018 Sep;116:1153-1162. doi: 10.1016/j.ijbiomac.2018.05.119. Epub 2018 May 18.

Genome sequence analysis of a novel Bacillus thuringiensis strain BLB406 active against Aedes aegypti larvae, a novel potential bioinsecticide.

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Laboratory of Biopesticides, Centre of Biotechnology of Sfax, P.O. Box "1177", 3061 Sfax, University of Sfax, Tunisia. Electronic address:
Laboratory of Bioinformatics, Biomathematics and Biostatistics, Institut Pasteur de Tunis, University of Tunis El Manar, 13, place Pasteur BP 74, 1002 Tunis, Tunisia.
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, P.O. Box "1177", 3061 Sfax, University of Sfax, Tunisia.


BLB406 is a novel isolate of Bacillus thuringiensis with a larvicidal activity against Aedes aegypti larvae. It displays original plasmidic and crystal protein patterns. The present work reported molecular and bioinformatic analyses for the genome sequence of BLB406 using MiSeq Illumina next-generation sequencing technology. The reads were assembled by Velvet tool. Using RAST program and PGAAP, the genome of BLB406 strain was shown to contain 6297 genes corresponding to 5924 protein coding sequences. The BLB406 genome investigation with BtToxin_scanner program shows that this strain has an original and different combination of toxins compared to the published ones: five cry genes (cry11, cry22, cry2, cry60, cry64) and two distinct vegetative insecticidal vip4 genes. This combination provides a potential larvicidal and anti-cancer activities to BLB406. It might be a potential solution to some problems such as the narrow insecticidal spectra and insect resistance. The whole BLB406 genome information provides a valuable background for future in silico analyses as well as biotechnological applications in order to increase the production of commercial bioinsecticide based on BLB406 B. thuringiensis strain.


Bacillus thuringiensis; Cry toxins; Genome sequencing and annotation; Larvicidal activity; Vegetative insecticidal protein Vip4

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