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Opt Lett. 2013 Jun 1;38(11):1860-2. doi: 10.1364/OL.38.001860.

Auto-aligning stimulated emission depletion microscope using adaptive optics.

Author information

1
Department of Cell Biology, Yale School of Medicine, 333 Cedar Street, New Haven, Connecticut 06510, USA. travis.gould@yale.edu

Abstract

Stimulated emission depletion (STED) microscopy provides diffraction-unlimited resolution in fluorescence microscopy. Imaging at the nanoscale, however, requires precise alignment of the depletion and excitation laser foci of the STED microscope. We demonstrate here that adaptive optics can be implemented to automatically align STED and confocal images with a precision of 4.3 ± 2.3 nm.

PMID:
23722769
PMCID:
PMC3749882
[Indexed for MEDLINE]
Free PMC Article

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