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J Infect. 2015 Nov;71(5):526-33. doi: 10.1016/j.jinf.2015.07.009. Epub 2015 Aug 5.

Assessment of CD27 expression as a tool for active and latent tuberculosis diagnosis.

Author information

1
Translational Research Unit, Department of Epidemiology and Preclinical Research, "L. Spallanzani" National Institute for Infectious Diseases (INMI) IRCCS, Via Portuense 292, Rome 00149, Italy.
2
Department of Epidemiology and Preclinical Research, INMI, Rome, Italy.
3
Clinical Department, INMI, Rome, Italy.
4
Translational Research Unit, Department of Epidemiology and Preclinical Research, "L. Spallanzani" National Institute for Infectious Diseases (INMI) IRCCS, Via Portuense 292, Rome 00149, Italy. Electronic address: delia.goletti@inmi.it.

Abstract

There are still no reliable tests to distinguish active tuberculosis (TB) from latent TB infection (LTBI). Assessment of CD27 modulation on CD4⁺ T-cells has been suggested as a tool to diagnose different TB stages.

OBJECTIVES:

To use several cytometric approaches to evaluate CD27 expression on Mycobacterium tuberculosis (Mtb)-specific CD4⁺ T-cells to differentiate TB stages.

METHODS:

55 HIV-uninfected subjects were enrolled: 13 active TB; 12 cured TB; 30 LTBI. Whole blood was stimulated with RD1-proteins or Cytomegalovirus-lysate (CMV). Interferon (IFN)-γ response was evaluated by cytometry. The proportion of CD27(±) within the IFN-γ⁺ CD4⁺ T-cells or RATIO of the CD27-median fluorescence intensity (MFI) of CD4⁺ T-cells over the CD27 MFI of IFN-γ⁺ CD4⁺ T-cells was evaluated.

RESULTS:

The greatest diagnostic accuracy in discriminating active TB vs. LTBI or cured TB was reached by evaluating the CD27(+) CD45RA(-) cells within the IFN-γ⁺ CD4⁺ T-cell subset (76.92 sensitivity for both, and 90% and 91.67% specificity, respectively), although the use of the CD27 MFI RATIO allows for stricter data analysis, independent of the operator.

CONCLUSIONS:

the study of CD27 expression using different approaches, whether it involves evaluation of CD45RA expression or not, is a robust biomarker for discriminating TB stages.

KEYWORDS:

Biomarker; CD27; Diagnosis; LTBI; Tuberculosis

PMID:
26253021
DOI:
10.1016/j.jinf.2015.07.009
[Indexed for MEDLINE]

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