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Int J Rheum Dis. 2018 Dec;21(12):2071-2078. doi: 10.1111/1756-185X.13381. Epub 2018 Dec 16.

Allergy inhibitory receptor-1 inhibits autoantibody production via upregulation of apoptotic debris clearance by macrophages.

Author information

1
Department of Internal Medicine, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
2
Department of Immunology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
3
Research Center of Immunology, ONO Pharmaceutical Co. Ltd., Osaka, Japan.

Abstract

AIM:

Allergy inhibitory receptor-1 (Allergin-1) is a newly identified immune regulatory molecule thought to influence autoantibody production. Autoantibody production, like that observed in Allergin-1-deficient mice, is crucial in the pathogenesis of several autoimmune diseases such as systemic lupus erythematosus. The purpose of this study is to clarify the regulatory role of Allergin-1-mediated autoantibody production using a murine model of thymocytic anaphylaxis.

METHODS:

C57BL/6 (WT) and Allergin-1-deficient mice were treated with apoptotic cells from naive thymocytes stimulated by dexamethasone. Antibody titers of total or immunoglobulin G (IgG) subclass of anti-double-stranded DNA (anti-dsDNA) and anti-histone antibody from serum were measured using an enzyme-linked immunosorbent assay. Macrophages from wild-type (WT) or Allergin-1-deficient mice were co-cultured with fluorescence-labeled apoptotic thymocytes or fluorogenic reagent and resultant phagocytic activity was quantified by with flow cytometry.

RESULTS:

After apoptotic cells injection, antibody titers of total and IgG3 anti-dsDNA and total anti-histone from serum were significantly increased in Allergin-1-deficient versus WT mice. Phagocytic activity was significantly lower in macrophages from Allergin-1-deficient mice versus WT mice.

CONCLUSION:

Allergin-1 might play an inhibitory role in autoantibody production via upregulation of macrophage phagocytosis.

KEYWORDS:

Allergin-1; autoantibody; lupus nephritis; phagocytosis; systemic lupus erythematosus

PMID:
30556363
DOI:
10.1111/1756-185X.13381
[Indexed for MEDLINE]

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