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Laryngoscope. 2018 Apr;128(4):E123-E129. doi: 10.1002/lary.26980. Epub 2017 Dec 14.

Use of autologous adipose-derived mesenchymal stem cells for creation of laryngeal cartilage.

Author information

1
Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana.
2
Department of Biomedical Engineering, Purdue University, West Lafayette, Indiana.
3
Department of Biomedical Engineering, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana.
4
Department of Speech and Hearing, Indiana University, Bloomington, Indiana, U.S.A.

Abstract

OBJECTIVES/HYPOTHESIS:

Adipose-derived mesenchymal stem cells (ASCs) are an exciting potential cell source for tissue engineering because cells can be derived from the simple excision of autologous fat. This study introduces a novel approach for tissue-engineering cartilage from ASCs and a customized collagen oligomer solution, and demonstrates that the resultant cartilage can be used for laryngeal cartilage reconstruction in an animal model.

STUDY DESIGN:

Basic science experimental design.

METHODS:

ASCs were isolated from F344 rats, seeded in a customized collagen matrix, and cultured in chondrogenic differentiation medium for 1, 2, and 4 weeks until demonstrating cartilage-like characteristics in vitro. Large laryngeal cartilage defects were created in the F344 rat model, with the engineered cartilage used to replace the cartilage defects, and the rats followed for 1 to 3 months. Staining examined cellular morphology and cartilage-specific features.

RESULTS:

In vitro histological staining revealed rounded chondrocyte-appearing cells evenly residing throughout the customized collagen scaffold, with positive staining for cartilage-specific markers. The cartilage was used to successfully repair large cartilaginous defects in the rat model, with excellent functional results.

CONCLUSIONS:

This study is the first study to demonstrate, in an animal model, that ASCs cultured in a unique form of collagen oligomer can create functional cartilage-like grafts that can be successfully used for partial laryngeal cartilage replacement.

LEVEL OF EVIDENCE:

NA. Laryngoscope, 128:E123-E129, 2018.

KEYWORDS:

Larynx; adipose stem cell; cartilage; chondrocytes; collagen; oligomer; tissue engineering

PMID:
29238978
DOI:
10.1002/lary.26980
[Indexed for MEDLINE]

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